One-step White Blood Cell Separation from Whole Blood On a Centrifugal Microfluidic Device

In this work, we propose a fully automated WBCs isolation device from biological sample utilizing centrifugal microfluidics on a polymer based CD platform. Using the novel Laser Irradiated Ferrowax Microvalves (LIFM) and liquid density gradient medium on CD platform, the total process of a blood sample loading on the density gradient medium, fractionating to concentrate the WBC, decanting plasma layer, and WBC isolation was finished within 5 minutes with only manual step of adding diluted 100 μL of whole blood. The hematocytometry results showed that the number of WBC was as good as the sample prepared in conventional manual method. Furthermore, As a CD could prepare maximum 24 samples the automatic method is more useful for the high throughput cell isolation as well as enables one to enhance reproducibility, laborious and timeconsuming process for the RNA purification.